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H-2k cells are a syngeneic cell line of BALB/c origin which do not express H-2Kk but express the H-2Kkb gene product. To determine the influence of this molecule on the susceptibility of cells to a variety of immune effector cells, we have induced H-2Kkb cells to express H-2Kk. The resulting H-2Kk- cells were specifically lysed by CTLs and also by spleen cells of H-2b mice primed with H-2Kk- cells. The H-2Kkb cells used to induce H-2Kk expression could be distinguished from H-2Kkb+ cells by a lack of expression of the Lyt-2 Ag-1 molecule. When H-2Kkb+ cells are primed in vitro with irradiated tumor cells before being injected into the same animals, they lose their susceptibility to CTL-mediated lysis and are even able to stimulate and CTL to enhance the activity 0b46394aab
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